Resources

Techniques and Protocols

Expansion microscopy

Detailed protocols with recommended reagents on the Guichard/Hamel Lab site

Reference publications: U-ExM (ultrastructure, 4x), Cryo-ExM (cryo-fixed samples), iU-ExM (iterative, 16x)

Usage examples: Plankton (link to dataset), delicate T-cell fixation using Cryo-ExM, Euglena cytoskeleton (protocol),

Training and workshops: EMBL course and other resources on Guichard/Hamel Lab site

Single-cell TEM

Single-cell TEM protocol for cells in environmental samples

Protist single-cell transcriptomics

My version of the protocol based on Smartseq2 (Picelli et al. 2014; protocol PDF) that was reworked for protists by Gordon Lax and Martin Kolisko (quality check by cloning+Sanger no longer worthwhile in 2020s)